Decellularization of liver tissues.

<p>(A) Schematic representation of the two-step decellularization protocol; for details see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0151736#sec002" target="_blank">Methods</a> section. (B) Representative images of human and murine liver tissues during the decellularization process at the indicated times. Scale bars: 5 mm. (C) Western blot analysis of supernatants for the indicated proteins. For each gel lane, 10 μg of protein sample were loaded; albumin was used as plasma and cellular protein markers; calnexin was used as a cellular protein marker. The lack of calnexin in supernatant 3 indicates the removal of plasma and cellular proteins from the scaffold. One representative experiment out of three is shown. (D) Percentage of ECM category components of human liver scaffold. The pie chart displays the results of one human decellularized liver biopsy with low fibrosis processed through the proteomics workflow. For each identified protein, protein abundance was calculated using the frequency of tandem mass spectra assigned to that protein.