Equivalency of androgen (AR BioAssay) and T (MS) measurements in urine of T-treated patients.

<p>AR BioAssay reporter cells were grown in 39.5 μl androgen-depleted culture media and 0.5 μl of glucuronidase-treated urine. The AR-YFP fluorescence level activated by androgens in the urine was compared against the T standard curve to extrapolate androgen concentration in T-equivalent activity levels. That concentration was multiplied by the 80-fold dilution of the urine in the cell culture media. These urine androgen levels (x-axis) then were compared to T measurements conducted by MS on the same samples (y-axis). For T-supplemented individuals, linear regression would be hypothesized to show equivalency (slope = 1.0) of androgen and T levels.