Large-scale purification of <i>Alpo</i>1, <i>Alpo</i>4 and <i>Alpo</i>6.
- Eveline Wijckmans (686705)
- Mieke Nys (686704)
- Sarah Debaveye (2575834)
- Marijke Brams (226304)
- Els Pardon (206973)
- Katrien Willegems (2575831)
- Daniel Bertrand (416984)
- Jan Steyaert (58922)
- Rouslan Efremov (2575837)
- Chris Ulens (226330)
Publication date
March 2016
DOIAbstract
<p>SEC-profiles of purified <i>Alpo</i> CLR homologues and SDS-PAGE analyses of the corresponding oligomeric peak fractions. Left: Purification of Alpo1-eGFP and <i>Alpo</i>1-wt in DDM. Middle: Purification of <i>Alpo</i>4-eGFP and <i>Alpo</i>4-wt in LMNG-CHAPS. Right: Purification of <i>Alpo</i>6-eGFP and <i>Alpo</i>6-wt in the presence of glycine and LMNG.</p
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