Pro-inflammatory cytokines downregulate 5-MTP production.

<p>(A) Measurement of 5-MTP in HUVEC-CM by ELISA. HUVECs were treated with VEGF or pro-inflammatory cytokines for 24 hours. H₂O denotes deionized water, Medium, control medium and cont, vehicle control. * indicates <i>P</i> < .05 compared to control. (B) Cell vitality is not affected by vascular permeability factors. Cell survival was analyzed by MTT assay. (C) Analysis of TPH-1 and TPH-2 transcripts in HUVECs treated with VEGF or pro-inflammatory cytokines for 24 hours. TPH-1 and TPH-2 transcripts were measured by RT-PCR. Upper panel shows a representative gel and the lower panel, densitometry of three experiments. Y79 retinoblast cells were included as reference. Cont indicates vehicle control. Error bars denote mean ± SD of three experiments. * indicates <i>P</i> < .05 compared to control.