D-Serine and Glycine Differentially Control Neurotransmission during Visual Cortex Critical Period - Fig 1

<p>D-serine modulates synaptic NMDARs current at VC L5PyNs <b>A:</b> Immunofluorescence for D-serine, and GFAP revealed that D-serine is expressed across all layers of P22-25 (5 slices, 3 animals) VC. The D-serine producing enzyme serine racemase (SR) was also found to be expressed and co-localized with the astroglial marker GFAP. Images are Z-stack of 10 serial confocal images with a thickness of 1μm. Scale bars: left, 200 μm; right, 50μm <b>B-D:</b> Applications of the D-serine degrading enzyme <i>Rg</i>DAAO (0.2 U/ml) reduces synaptically evoked NMDA-EPSCs (n = 5 cells, 5 slices, 2 animals) (B) while its inactive form Δ<i>Rg</i>DAAO has no effect (n = 5 cells, 5 slices, 2 animals) (D). Scale bars: 100pA, 500ms. Conversely D-serine (100 μM) significantly potentiates NMDA-EPSCs (n = 5 cells, 5 slices, 2 animals) (C) Scale bars: 200pA, 500ms. ***p<0.001.