AFM Imaging Reveals Topographic Diversity of Wild Type and Z Variant Polymers of Human α₁-Proteinase Inhibitor - Fig 4

<p><b>Volume distributions of WT α</b>_<b>1</b><b>-PI (A) and Z variant (B) particles.</b> The distributions revealed a prevailing content of objects, which size was consistent with monomers. Surprisingly, the presence of larger molecules was also detected. Among the larger objects, particles with volume approximately twice as big as monomers were the most abundant. The content of dimers and bigger particles was more pronounced in the Z variant (B) than in the wild type (A). The volume of particles was calculated with the grain analysis of SPIP software. Relative count of particles is shown. Total of 847 and 639 particles were analyzed for the WT and Z variant, respectively. Histogram bin size was 5 nm. Fitted normal distribution curves are shown in green for monomers and in blue for dimers, whereas total frequency distribution is represented by red traces (OriginPro).