Inhibition of inward TRPM7 current by IL-6/sIL-6R in WT HEK293-TRPM7 cells.

<p>(A) Typical TRPM7 currents in WT HEK293-TRPM7 cells treated with 20 ng/mL IL-6 combined with 25 ng/mL sIL-6R in divalent ion-free extracellular solution. (B) Inhibition of TRPM7 inward, but not outward currents, in WT HEK293-TRPM7 cells by IL-6/sIL-6R. All current values were normalized to an inward current value of controls at +100 mV. (n = 6, * p < 0.05). (C) Rapid and reversible inhibition of TRPM7 currents by IL-6 combined with sIL-6R. (D) Dose-dependent reduction of the TRPM7 currents at -80 mV by 8 ng/mL IL-6 combined with 10 ng/mL sIL-6R, 40 ng/mL IL-6 combined with 50 ng/mL sIL-6R, or 200 ng/mL IL-6 combined with 250 ng/mL sIL-6R, respectively. (n = 9, * p < 0.05 vs. control, # p < 0.01 vs. control). (E) A typical TRPM7 current in TRPM7-HEK293 cells perfused with normal extracellular solution. (F) Inhibition of TRPM7 currents by IL-6/sIL-6R in normal extracellular solution in TRPM7-HEK293 cells.