Decreased deposition of extracellular fibronectin in iHTM cells after PGA treatment.

<p>Confluent cell cultures were treated with 0.1% DMSO (Control), 10 μg/ml bimatoprost (Bima) or 10 μg/ml latanoprost (Latan) continuously for 5 days. Cells were then labeled with antibody against fibronectin and IgG conjugated with FITC successively. A: Phase-contrast micrographs showing the morphology of iHTM cells treated with PGA or vehicle DMSO. B: Representative photomicrographs showing fibronectin immunostaining. Scale bar, 20 μm. C: Quantification of fluorescence intensity. Fluorescence intensity was measured using IPP 6.0 System to determine the means integrated option density to express the relative protein level. The results were expressed as <i>means±SD</i> (<i>n</i> = 3). *, <i>p</i><0.05, **, <i>p</i><0.01, compared with control.