Comparative analyses of DenA protein levels in Δ<i>dipA</i>, Δ<i>csnG</i> and Δ<i>dipA</i>/Δ<i>csnG</i> double mutant strains.

<p><b>(A)</b> DipA destabilizes DenA during illumination which induces asexual development in wild type. Western hybridization showed a stable DenA-GFP in wild type background at vegetative growth (Veg.) and an unstable protein during illumination induced asexual development (Asex). DenA-GFP in a Δ<i>dipA</i> strain is stable under the same conditions. <b>(B)</b> Top and bottom view of illuminated wild type colony (WT) developing asexual spores and <i>dipA</i>, <i>csnG</i> and combined <i>dipA</i>/<i>csnG</i> deletion strains under the same conditions. Equal amount of spores were point-inoculated and incubated for three days during illumination. <b>(C)</b> DenA-GFP protein stability in WT and <i>dipA/csnG</i> deletion background without the potential to produce asexual spores during illumination (Asex.). <b>(D)</b> Diagram of DenA-GFP protein levels in Δ<i>csnG</i>, Δ<i>dipA</i> and <i>dipA</i>/<i>csnG</i> double deletion strain from three independent experiments during vegetative growth (Veg.) and illumination (Asex.) for indicated time points. SDS gels were loaded with equal amounts of protein crude extract. Membranes were treated with GFP-antibody and stained with Ponceau as loading control and the GFP/Ponceau pixel ratio was calculated. The standard deviations are shown. DenA-GFP: 54.5 kDa and free GFP: 25 kDa.