Ft elicts recruitment of IMC with MDSC phenotype.

<p>(A) Flow plots of multiple myeloid cell analysis scheme in in Ft LVS-infected lungs. Ft-infected lung cells were stained for cell-specific markers (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005517#sec009" target="_blank">Methods</a>); lymphoid cells were excluded and cells in R1 gate were analyzed for CD11b and CD11c expression. (B) Cell numbers of myeloid subsets from LVS or SchuS4 infected mice were compared with control mice (0 dpi) using gating strategy as shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005517#ppat.1005517.s004" target="_blank">S4A–S4G Fig</a>(mean ± SD of two (SchuS4, n = 6 mice) or three (LVS, n = 9 mice) independent experiments, Student’s t-test, *p<0.05 and **p<0.01 indicate differences at various dpi). (C) Cells from R4 gate were analyzed for F4/80, Ly6G and Ly6C expression. (D) Cell numbers of Ly6G⁺ and Ly6C⁺ cells from F4/80^- population in LVS or SchuS4 infected mice (mean ± SD of two (SchuS4, n = 6 mice) or three (LVS, n = 9 mice) independent experiments, Student’s t-test, *p<0.05 and **p<0.01 indicate differences at various dpi). (E) Cell numbers of F4/80⁺ and F4/80^- cells (R4 gated) in lungs following lethal (1000 cfu) or sub-lethal (500 cfu) LVS infection (mean ± SD of two experiments, Student’s t-test, *p<0.05 indicates a difference between LD₁₀₀ and LD₅₀).