Localizations of epitope tagged proteins of genes in consensus between transcriptional studies.

<p>Proteins tagged with HA or 3xHA (red) were visualized in conjunction with CWP1 (green) and DAPI stained DNA (blue) for trophozoites, 7 and 22 h post induction of encystation and cysts. (A) The hypothetical protein 10552-3xHA displayed an ER-like localization in a punctuate manner at both 7 and 22 h. (B) 12082-3xHA appears in vesicle like compartments in early encysting cells and ER like localization in late encysting cells. (C) 102813-3xHA is annotated as a Protein 21.1 and localizes to the nuclei in a proportion of the cells in the population, but not in cysts. (D) 103785-HA localizes to the ER and non-ESV, vesicle-like structures during encystation and in cysts. Scale bars represent 10 μm.