Exercise Inhibits Adipose Progenitor Proliferation and Differentiation.

<p>(A) Fluorescent microscopy of inguinal (IWAT) and perigonadal (PGWAT) white adipose depots after two months of indicated treatment. Two-second exposure used in order to visualize GFP in groups that received Dox (+Dox). (B) Percentage of GFP-positive (GFP+) cells in the subcutaneous stromal vascular fraction (SVF) of mice two months after indicated treatments (Sed: sedentary, Ex: exercise). (C) Percentage of GFP+ cells that are BrdU-positive (BrdU+) in the subcutaneous SVF of mice two months after indicated treatments. BrdU was given in the drinking water for two days prior to analysis. (D) Percentage of GFP+ nuclei in adipocyte fraction isolated from subcutaneous fat depots two months after indicated treatment. (E) Oil Red O staining of SVF from subcutaneous fat plated two months after indicated treatment. Cells were plated to confluency and stained four days post insulin induction. Representative studies on P120 males; n ≥ 12 per cohort, repeated ≥ 3 cohorts. Error bars indicate SEM. Statistical significance assessed by two-tailed Student’s t-test. *p<0.05, **p<0.01.