Performing of bipedicled flap induced impaired granulation tissue formation and wound healing.

<p>(A) Representative H&E stained sections showing thinner granulation tissue thickness in ischemic wounds at day 5 after wounding. Scale bars indicate 500μm. (B) Data was obtained from 12 wounds (n = 6), and shown as the mean value ± SD. Statistics are shown as comparisons between the non-ischemic wounds and ischemic wounds. **<i>p<0</i>.<i>01</i>. (C) Masson-trichrome staining showing collagen deposition of non-ischemic and ischemic wound at day 5 and 11 after wounding. (D) Collagen deposition rate were calculate using IPP software. Data was obtained from 12 wounds (n = 6), and shown as the mean value ± SD. **<i>p<0</i>.<i>01</i>. (E) Representative H&E stained sections and immunohischemistry stained with anti-PECAM-1 antibody showing angiogenesis of both non-ischemic and ischemic wounds at day 5 after wounding. Scale bars = 200μm.