Performing of bipedicled flap induced ischemia and impaired re-epithalialization and wound healing.

<p>(A) 80mins before animals were scarified, pimonidazole hydrochloride adducts solutions were intraperitoneal injected at a dosage of 60mg/kg BW. The wound tissues were then harvested. Pimonidazole hydrochloride adduct were determined with an anti-pimonidazole antibody using immunohischemistry method. Scale bars = 200μm. (B) Wounds size were record at day 1, 5, 9, 13 after wounding using transparent tracing paper. Healing rate of wounds was calculated. Data are shown as the mean ± SD. **<i>p<0</i>.<i>01</i>. (C) Representative H&E stained sections showing shorter migrating tongue in ischemic wounds at day 5 and 11 after wounding. Scale bars indicate 500 μm. (D) Data was obtained from 8 wounds (n = 4), and shown as the mean value ± SD. Statistics are shown as comparisons between the non-ischemic wounds and ischemic wounds. **<i>p<0</i>.<i>01</i>. The proliferation zone was highlighted in yellow line and the migration tongues were depicted in red line.