Changes in viable cell numbers and biofilm formation by <i>P</i>. <i>aeruginosa</i> PA01 and <i>S</i>. <i>aureus</i> Xen 30 in the presence of copper particles electrospun into nanofibers (Cu-F) and nanofibers without copper particles (CF).

<p>Cells not treated with copper and not cultured in the presence of nanofibers serves as control (labelled c). Incubation was at 37°C. Readings were taken at 3, 6, 24 and 48h. (a) Number of viable cells of <i>P</i>. <i>aeruginosa</i> PA01, (b) total biofilm formation by cells of <i>P</i>. <i>aeruginosa</i> PA01, (c) number of viable cells of <i>S</i>. <i>aureus</i> Xen 30 and (d) total biofilm formation by cells of <i>S</i>. <i>aureus</i> Xen 30. Biofilm formation was determined by staining with crystal violet and recording OD readings at 595 nm. Data points presented are the average of three independent experiments (mean ± standard deviation). * <i>p< 0</i>.<i>05</i>.