Fold change in gene expression of Th17, Treg, Th1 and 2 associated markers in leprosy reaction patients (RR,ENL) as compared to matching stable leprosy patients (BT, LL) as evaluated by qPCR of <i>ex-vivo</i> antigen (MLSA) stimulated PBMC.

<p>Horizontal histogram shows significant fold increase in (a) RR, (b) ENL as compared to BT, LL patients respectively for gene expression of Th17 and Treg associated cytokines, receptors and transcription factors as well as Th1 and Th2 cytokines. p value was calculated based on a Student’s t-test and p< 0.05 was considered to be statistically significant. Abbreviations: BT; borderline tuberculoid, RR; reversal reaction, LL; lepromatous leprosy, ENL; erythema nodosum leprosum as per Ridley Jopling classification[<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004592#pntd.0004592.ref002" target="_blank">2</a>,<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004592#pntd.0004592.ref029" target="_blank">29</a>], ns = non significant.