MADD-2 expression in the primordium.

<p>(A) Wild-type embryos expressing MADD-2::GFP (green) and the general membrane reporter (red) (n = 32 embryos examined). The panels at right are high magnifications of the bracketed regions. (B) Diagram summarizing changes in MADD-2::GFP levels over time, ranging from high (dark green) to low (white). Note that MADD-2::GFP disappears in both L and R cells in the int1 ring after about 345 minutes, and remains detectable in both R and L cells in the int8 and int9 rings until at least 600 minutes. MADD-2::GFP expression was not detected in the dorsal midline valve cell, v3D, which rotates counterclockwise (345 minutes), but was detectable in non-intestinal cells that move dorsally to flank v3D (pm8 at 345 minutes, see [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005950#pgen.1005950.ref009" target="_blank">9</a>]). (C) MADD-2::GFP expression in an <i>apx-1(zu347ts)</i> mutant embryo following a shift to restrictive temperature (26°C) at the E8 stage. MADD-2::GFP expression disappeared in the int1 ring, as in wild-type embryos, but persisted in both R and L cells in the int2-4 rings. The image is of the ventral side of the intestine; the int2-int4 rings did not rotate, so both R and L cells are visible at the ventral midline. Panels = A,B [JJ2536], C [JJ2537]. Bar = 5 microns.