Effects of <i>Mdm2</i>^<i>C305F</i> mutant on selected hematopoietic lineage subpopulation in bone marrow.

<p>(A) Representative flow cytometry analysis of the specific precursor populations in control (n = 7), <i>Mdm2</i>^<i>C305F</i> homozygous (n = 6), and <i>Mdm2</i>^{<i>C305F/C305F</i>};<i>Trp53</i>^<i>ko/ko</i> mice (n = 6). (B) <i>Mdm2</i>^<i>C305F</i> mutant animals exhibited a significant paucity in the HSPC population of LSKs (<i>P</i> < 0.01), accompanied by significant decreases of MPP populations (<i>P</i> < 0.01). HSPC indicates hematopoietic stem and progenitor cell; LSK, Lin^-Sca-1⁺c-Kit⁺; LT-HSC, long-term hematopoietic stem cells; ST-HSC, short-term hematopoietic stem cells; MPP, multipotent progenitor; CLP, common lymphoid progenitor; and CMP, common myeloid progenitor.