AJ and TJ disruption in ADAM10 KD embryos.

<p>(A) Altered expression of genes related AJ and TJ formation (<i>ADAM17</i>, <i>CXADR</i>, <i>TJP1</i>, <i>Cdh1</i>, <i>CLDN6</i>, <i>OCLN</i>, and <i>TFAP2C</i>). (B) FITC-Dextran uptake assay for paracellular permeability. ADAM10 KD blastocysts showed defects of paracellular sealing compared to the control. (C) Representative images of FTIC-dextran treated embryos under bright field and U.V. Scale bars: 200 μm. (D) AJ and TJ protein localization in ADMA10 KD embryos by ICC. In the KD blastocysts, TJ proteins (CXADR, TJP1, and OCLN) and the AJ protein (CDH1) were not localized to the apical region or reduced. Asterisks indicate statistically significant differences (<i>P</i> < 0.05). The data are presented as mean ± SEM. RQ: relative quantification. Scale bars: 50 μm.