Increased total PKCε levels in DIO islets.

<p>Proteins from ND, LDR and HDR islets were probed with an antibody against total PKCε (T-PKCε) and phospho-Ser⁷²⁹ PKCε (P-PKCε). Tubulin or total PKCε were used for normalization. (A) Representative western blot of T-PKCε and P-PKCε, (B) expression level of T-PKCε normalized by tubulin, (C) P-PKCε level normalized by tubulin and (D) by total PKCε. Means ± SEM of 8 (ND), 13 (LDR) and 10 (HDR) mice. *p<0.05, **p<0.01 vs ND, One-way ANOVA, Tukey post-hoc test.