Effect of TNF and IL-10 deficiency on growth.

<p>A. Mean body weight is shown for female mice of the indicated genotypes from weaning until 28 weeks of age (n = 5 for T/I-het and T/I mice; n = 3–5 for WT, with 65 measurements per mouse). Although weights for all 3 genotypes were statistically similar at weaning, the weights of the T/I mice became significantly lower than their T/I-het littermates beginning at 8 wks and was lower than the WT mice beginning at 13 wks and they remained significantly lower for the remainder of the study (p < 0.05, 2 way ANOVA with Dunnett’s post-test). Similar trends were seen for male mice. SEMs averaged 0.8 g across all days and groups; error bars are omitted for clarity. B. Colitis histologic scores are shown for cohorts of 9–12 wk old mice. Each point represents a single mouse studied: WT (n = 11), <i>Tnf</i> ^-/- (n = 6), <i>Il10</i> ^-/- (n = 7), T-het/I (n = 17), T/I-het (n = 5), and T/I (n = 14). A score of ≤12 indicates absence of colitis, scores ≥ 25 indicate moderate to severe colitis, and the maximum possible score is 75. * indicates a significant difference from WT; p ≤ 0.0001 for both T-het/I and T/I (Kruskal-Wallis non-parametric test with correction for multiple comparisons).