Effect of vaccination with <i>Bm</i>-RAL-2 and <i>Bm</i>-103 individually, concurrently and as a fusion protein on the development of protective immunity manifested as % reduction in worm burdens and % host protection against infection with <i>Brugia malayi</i> L3s in Mongolian gerbils.

<p>(A) Vaccination with <i>Bm</i>-RAL-2 (42 dpi); (B) Vaccination with <i>Bm</i>-103 (42 dpi); (C) Vaccination with <i>Bm</i>-RAL-2—<i>Bm</i>-103 fusion protein or with <i>Bm</i>-RAL-2 + <i>Bm</i>-103 concurrently (90 dpi); (D) Vaccination with <i>Bm</i>-RAL-2—<i>Bm</i>-103 fusion protein or <i>Bm</i>-RAL-2 + <i>Bm</i>-103 concurrently (150 dpi). Each dot represents the total number of adult worms recovered from an individual animal. The lines represent mean with standard deviation. <i>P</i> ≤ 0.05 denotes a statistically significant difference in total worm recovery (percent reduction) between vaccinated and alum control group, Mann–Whitney U Test, GraphPad Prism 6.