Treatment induced survivin expression.

<p>Survivin and XIAP expression profiles in Y79 (A), CHLA-215 (B), and RPE (C) cells exposed to carboplatin (CBDCA, 5 μM), topotecan (TOPO, 10 nM), or ionizing radiation (Rad, 5 Gy) with or without concomitant exposure to the survivin suppressant Sepantronium bromide (YM155, 2 nM). Cells were seeded on day 1 in growth medium in the presence or absence of 2 nM YM155 and 24 hours later cells were treated with or without CBDCA, TOPO, or Rad. At 72 hours, cells were harvested for protein expression analysis. Top figures show a representative Western immunoblot and bottom figures provides quantitative densitometric values from all immunoblots. <i>Columns</i>, mean; <i>bars</i>, ± SE of three to five separate experiments. ** <i>P</i> < 0.05 (Control vs CBDCA, TOPO, or Rad); *<i>P</i> < 0.05 (Control, CBDCA, TOPO or Rad in the absence of YM155 vs Control, CBDCA, TOPO or Rad in the presence of YM155).