Zebrafish embryos injected with CPA6-MO2 and -MO4 are responsive to stimuli.

<p>Zebrafish embryos 3 dpf were stimulated by pushing 200 μl of the embryo media in which they were suspended towards them using a P-200 pipette at moderate pressure (A) or by touching with a hair (B). Water stimuli were calibrated to evoke a response only ~40% of the time under basal conditions while touch stimuli evoked a response 80% of the time. Statistical analysis was performed by one-way ANOVA. (C,D) PTZ (2.5 mM) was added to the embryo media and fish were tested at 5 minute intervals for responsiveness to the water stimulus. There were no statistically significant differences between embryos injected with Ctrl-MO, CPA6-MO2 or CPA6-MO4 either in basal conditions or in the presence of PTZ during the time periods examined for either stimulation paradigm. Statistical analysis was performed by repeated measures ANOVA. Error bars show SEM.