PTZ treatment affects mRNA levels in zebrafish embryos.

<p>Zebrafish embryos (wild-type 3 dpf) were exposed to 15 mM PTZ for 1 h and then transferred to drug-free embryo medium for 1, 5, or 23 h after which mRNA was extracted. Quantitative PCR was used to examine levels of various mRNAs. Error bars show SEM (n = 4 for each group). Statistical analysis was performed by Student’s <i>t</i> test: *, <i>p</i> < 0.05; **, <i>p</i> < 0.01; ***, <i>p</i> < 0.001 compared with Ctrl-MO group.