Induction of CD4⁺ T cell lines that are reactive to cancer antigens by CD14-ML-DC obtained from HNC patients.

<p>CD4⁺ T cells isolated from PBMCs of HNC patients were stimulated with CD14-ML-DC that were pulsed with a mixture of 6 kinds of peptides (CDCA1_39-64, CDCA1_55-78, KIF20A_60-84, KIF20A_809-833, LY6K_119-142 and LY6K_172-191). After more than three rounds of stimulation, the number of CD4⁺ T cells that reacted to each peptide was analyzed by ELISPOT assay. CD14-ML-DC were used as stimulators in the assay, because only a few amount of blood samples could be obtained from cancer patients. The results for cancer patient 1 (A) and cancer patient 2 (B) are shown.