<i>In Vivo</i> Energy Homeostasis of HFD-fed Cohort B Males.

<p>In HFD-fed 17 week-old mice (n = 6–7), (<b>a</b>) 24-h activity, with inset showing mean beam breaks across the first 4 h of the dark period (y-axis units of beam breaks), as well as (<b>b</b>) energy expenditure, (<b>c</b>) respiratory exchange ratio, and (<b>d</b>) food intake were averaged across 48–84 h, with grey indicating dark cycles. Energy expenditure is shown as estimated marginal means ± SEM, adjusted for covariates of lean and fat mass; other data are simple means ± SEM. Dark red represents HFD-fed <i>Ins1</i>^-/-:<i>Ins2</i>^+/+ male mice; orange represents HFD-fed <i>Ins1</i>^-/-:<i>Ins2</i>^+/- male mice. <i>p</i> ≤ 0.05 denoted by # for <i>Ins1</i>^-/-:<i>Ins2</i>^+/+ vs <i>Ins1</i>^-/-:<i>Ins2</i>^+/-.