Effect of different gene inactivations on <i>arsA</i> gene expression measured by RT-qPCR.

<p>The level of the <i>arsA</i> transcripts was measured under encystment inducing conditions and it was normalized according to the level of the <i>gyrA</i> mRNA. The data are presented as fold change of <i>arsA</i> mRNA levels in mutants with <i>gacA</i>, <i>rsmZ1</i>, <i>rsmA</i>, and <i>gacA</i>-<i>rsmA</i> gene inactivations (strains SW5, SW13, SW11 and SW15), relative to those of the wild type strain (SW136). Determinations were made from bacterial cultures grown for 36 h in liquid BBOH medium at 30°C. The data represent the mean of triplicates and the error bars represent standard deviations. Asterisks indicate statistical significance (unpaired Student's <i>t</i>-test) in the comparison of each mutant versus the wild type strain, *P < 0.05.