Leptin increases metabolic rate and raises RER.

<p>(A-D) Metabolic measurements were performed in OB^[Emp] (white bar, n = 5) and OB^[<i>Lep</i>] (black bar, n = 6) mouse groups in CLAMS metabolic cages at 28 days post injection. Metabolic data for OB^[3T3] group are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153198#pone.0153198.s003" target="_blank">S3 Fig</a>. The average (mean ± SD) x, y, and z activity (A), average VO₂ (mL/kg/h) (B), respiratory exchange ratio (RER) (C), and average metabolic rate (kcal/h/kg) (D) are shown for both the dark (D) and light (L) cycles. (E, F) Kinetic data for RER (E) and metabolic rate (kcal/h/kg) (F) are shown as mean for each time point in OB^[Emp] (open black circles) and OB^[<i>Lep</i>] (filled red circles) groups. Significant differences are shown by black bar and determined by one-way ANOVA followed by Tukey’s test.