Effect of silencing (A–E) and overexpressing (F–I) TSPO in HT-29 cells.

<p>(A) Time dependence of TSPO mRNA expression in control cells (open bars), after mock transfection (grey bars) or siRNA-targeting TSPO transfection (dashed bars). (B) HT-29 cell proliferation (controls, black circles; mock-transfected, grey squares; and siRNA-targeting TSPO transfected, grey triangles). Time course of mRNA expression of <i>TSPO</i> (C), <i>IL-8</i> (D), and <i>MnSOD</i> (E) relative to <i>GAPDH</i> after 3–6 hours, 24 hours, or 72 hours of treatment with 10 ng/mL of TNF performed 24 hours after transfection by siRNA-targeting TSPO (controls, open bars; siRNA alone, short dashed bars; TNF-treated, black bars; siRNA and TNF-treated, long dashed bars). (F) HT-29 cell proliferation (controls, black circles; mock-transfected, grey squares; empty vector plasmid, open squares; and TSPO cDNA-containing vector, grey triangles) in the presence of TNF (nontransfected cells or siRNA-targeting TSPO transfected cells). Time dependence of mRNA expression of <i>TSPO</i> (G), <i>IL-8</i> (H), and <i>MnSOD</i> (I) relative to <i>GAPDH</i> after 3–6 hours, 24 hours, or 72 hours of treatment with TNF performed 24 hours after transfection by vector plasmid (controls, open bars; cDNA alone, short dashed bars; TNF-treated, black bars; cDNA and TNF-treated, long dashed bars). The results are expressed as the means ± standard error of the mean.