Colocalization of TGF-β1, pepsin and macrophage and lymphocyte-positive cells in the tonsil sections and mRNA expression for inflammatory mediators in tonsil tissues with tonsillar hypertrophy.

<p><b>A)</b> Dual pepsin and CD68-positive cells and dual TGF-β1and CD68-positive cells were detected in the crypt. <b>B)</b> Dual pepsin and CD68-positive cells and dual TGF- β1and CD68-positive cells were also detected in the surrounding germinal centers. <b>C)</b> Few dual pepsin and CD20 or CD45-positive cells were detected. Scale bar, 50 μm. <b>D)</b> The mRNAs of IL-1, IL-6, and TNF- β in the tonsil tissues were determined by RT-PCR. GAPDH housekeeping gene was used as a control.