Detection of PAK isoforms and markers in human prostate tissue, and in WPMY-1 cells.

<p>Detection was performed by RT-PCR <b>(A)</b>, or Western blot analyses <b>(B, C)</b>. Shown are ratios of Ct values (target/GAPDH) for each sample (from n = 7 patients, or from WPMY-1 cells from 4 experiments) <b>(A)</b>, or bands for all investigated samples (from n = 12 patients, or WPMY-1 cells from 4 experiments <b>(B)</b>. In <b>(B) and (C)</b>, indicated molecular weights are the expected sizes of proteins. Detection of each PAK isoform in <b>(B)</b> was performed with two different antibodies for each isoform (mouse, and rabbit or goat) (ab, antibody). Western blots included calponin as a marker for smooth muscle cells, pan-cytokeratin as a marker of endothelial cells (glands), prostate-specific antigen (PSA) as a marker for hyperplasia, and α_1A-adrenoceptors as an important feature of prostate smooth muscle cells.