Overview of mass cytometry phenotyping in early AML therapy.

<p>(A) A timeline of AML induction shows blood and bone marrow collection goals for phenotypic comparisons as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153207#pone.0153207.g002" target="_blank">Fig 2</a>. Samples collected and analyzed for individual patients are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153207#pone.0153207.g003" target="_blank">Fig 3</a> and Figure A in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153207#pone.0153207.s001" target="_blank">S1 File</a>. (B) viSNE analysis of all live cells from the diagnosis marrow of one AML patient F004 is shown. Cells were arranged on the viSNE map along unitless x and y viSNE axes according to 27-dimensional phenotype (Table A in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153207#pone.0153207.s001" target="_blank">S1 File</a>) so that phenotypically similar cells were placed near each other. Cellular abundance is indicated with a shaded contour plot where outliers start at 10% and each 2% contour is shaded a lighter color from purple to yellow. (C) On the same viSNE axes as in (B), diagnostic bone marrow cells from patient F004 were graphed and shaded according to identity determined by immunophenotype. AML blast cells were shaded red and non-blast cells were shaded grey. (D) On the same viSNE axes as in (B), diagnostic bone marrow cells from patient F004 were graphed and shaded according to expression of CD45 on a rainbow heatmap (log-like arcsinh₁₅ scale).