The addition of a ubiquitination site to Icl1 confers <i>C</i>. <i>albicans</i> with Crabtree positive-like properties.

<p>(A) Western blot demonstrating that the addition of a ubiquitination site to a Myc₃-tagged CaIcl1 protein leads to its destabilization following glucose exposure in the <i>C</i>. <i>albicans</i> clinical isolate SC5314. Similar data were obtained in two independent replicate experiments. (B) Resistance of <i>C</i>. <i>albicans</i> strains to 2-deoxyglucose (2DG) when growing on lactate (Lac) or glucose (Glu). Cultures were replicated onto Glu and Glu + 2DG (growth examined at 2 days), Lac (growth at 4 days), and Lac + 2DG (growth at 7 days): WT, SC5314; <i>icl1Δ/Δ</i>, DCY65, <i>ICL1</i>, DCY75 (<i>ICL1-Myc</i>_<i>3</i><i>-NAT1</i>); <i>ICL1-Ubi</i>, DCY82 (<i>ICL1-UbiMyc</i>_<i>3</i><i>-NAT1</i>) (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005566#ppat.1005566.s007" target="_blank">S2 Table</a> in the supplementary information).