Slower average cell cycle progression and loss of synchrony in gonads with reduced reproductive activity.

<p>(A, B) Principle of EdU pulse chase analysis. Three fictitious mitotic zones cycle steadily (A) or stochastically enter a dormant state (B; play and pause symbols within the squares). The position of each square on the circle represents mitotic zone cell cycle progression (progression from time of labeling highlighted by a colored band). A full revolution on the circle corresponds to all cells in the mitotic zone having undergone a full cycle. The red wedge in the bottom row shows average cycle progression (angle shown by red arrows) and the amount of dispersion between mitotic zones (width of the wedge, set to the inverse of the magnitude of the resultant computed as the vector sum of individual gonad positions). (C) Analysis of cell cycle progression after EdU pulse-chase. Mitotic zones from virgin females or old wild-type hermaphrodites lose synchrony at later chase times, as shown e.g. by wider wedges (virgin fog-2 data is repeated in rows 2 and 3 to facilitate comparisons). See also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005985#pgen.1005985.s005" target="_blank">S5 Fig</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005985#pgen.1005985.s011" target="_blank">S5 Table</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005985#pgen.1005985.s016" target="_blank">S1 Movie</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005985#pgen.1005985.s015" target="_blank">S1 Dataset</a> and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005985#pgen.1005985.s017" target="_blank">S1 Text</a>.