HCMV replication is enhanced by 9-HODE treatment.

<p>(A) Representative immunofluorescence results of IE expression in NSCs infected by HCMV at a MOI of 10 in the presence of increasing concentrations of 9-HODE or the vehicle, 48 h pi. (B) Immunofluorescence analysis showing increasing number over time of IE positive NSCs in cultures infected by live HCMV at a MOI of 10 in the presence of increasing concentrations of 9-HODE or the vehicle, 48 h pi. Data represent means ± CI of 2 independent experiments, each being performed in duplicate. NI: uninfected control. (C) Titration of the viral particles in supernatants of MRC5 fibroblast cultures treated beforehand with supernatants of NSCs infected by live HCMV at a MOI of 10 in the presence of 1 μg/ml 9-HODE or the vehicle, 48 h pi, or uninfected NSCs. HCMV strain was AD169. Data represent means ± CI of 2 independent experiments, each being performed in triplicate. *: p< 0.05; **: p< 0.01.