Changes in Pol II profile, gene expression, and poising index upon B cell activation.

<p>a) Violin plots comparing distributions of fold changes of Pol II promoter density (left) and Pol II gene body density (right) for two groups genes: genes that continued to be poised after B cell activation (6802 genes) and genes that switch from poised to non-poised class during B cell activation (1138 genes). The y-axes are in a logarithmic scale. The p-values from Mann Whitney U test and Cohen's d effect sizes that reflect the differences between two groups are reported on top. b) Violin plots comparing the distributions of fold changes of poising index and mRNA expression in two groups of genes based on their change in transcriptional activity along gene body upon B cell activation: genes with similar Pol II body density (2/3 < fold change < 1.5; 6069 genes) and genes with large increase of Pol II body density (fold change > 1.5; 1491 genes) between RESTB and ACTB. c) The Spearman and partial Spearman correlations between changes in gene expression and changes in Pol II promoter/gene body density during the transition from RESTB to ACTB for common 8769 Pol II⁺ genes.