Additional file 3: Figure S2. of Influence of platelet-activating factor receptor (PAFR) on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortus

PAFR activation-related F-actin polymerization for phagocytosis of B. abortus with different infection doses. RAW 264.7 cells were pretreated with or without PAF (200 nM) for 5 min, followed by infection with B. abortus (MOI 10, 50 and 100) for 5 (a) and 30 (b) min, and then cells were subjected to the FACS analysis for F-actin content. (c) The quantitative analysis results of experiment in (a-b). Data represent the mean ± SD of triplicate trials from three independent experiments. Statistically significant differences from the infected cells with B. abortus (MOI 10) are indicated by asterisks (*, P <0.05, **, P < 0.01, ***, P < 0.001). (TIF 895 kb