Expression of CIP2A by murine lymphoid organs does not impact the immune system of unchallenged CIP2A^HOZ animals.

<p>(A) Semi-quantitative RT-PCR for CIP2A RNA in main lymphoid organs and testis from WT and CIP2A^HOZ adult mice. Average expression from at least 3 mice (despite testis; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0152996#pone.0152996.s006" target="_blank">S1 Table</a>). (B) Protein expression of spleen and testis from WT and CIP2A^HOZ adult mice. Mouse beta-actin was used for normalization. (C) CIP2A-IHC and hematoxilin-eosin staining of common lymphoid organs from adult wild-type mice. Asterix indicate CIP2A expression in germinal centers of lymph nodes and spleen. (D) Hematoxilin and eosin immunohistological staining reveals normal morphology of lymphoid tissues in control and CIP2A^HOZ mice. Thick scale bar: 100 μm (Bone marrow); 250 μm (Spleen); 1 mm (Thymus). (E) Flow cytometry analysis of frequencies of leukocyte subpopulations in peripheral blood of WT or CIP2A^HOZ females and males (10 mice per condition). (F) Principal immunoglobulins content (μg/ml; except for IgE, ng/ml) in the peripheral blood of WT or CIP2A^HOZ adult mice (*: 10 mice per condition, except for IgE, with samples from 9 WT or CIP2A^HOZ females, 9 WT males and 7 CIP2A^HOZ males). (E, F) Medians are represented and p-values calculated based on a Wilcoxon rank-sum test.