<p>Serial dilutions of yeast cells (cells ml<sup>-1</sup>) that were transferred with the prey and bait constructs shown in the figure were determined for growth on yeast minimal synthetic defined base (SD) lacking tryptophan, leucine and histidine. The plasmid pairs pGADT7 and GBKT7-53 was used as a positive control, and the plasmid pairs pGADT7 and pGBKT7-Lam and was used as a negative control.</p
<p>The yeast strain YH2Gold containing the indicated plasmid combinations was grown on nutritional s...
<p>Transformants containing the bait and prey plasmids were visualized by their growth on double dro...
<p>The pGBKT7-TaCBLs and pGADT7-TaCIPK2 plasmids were co-transformed into the AH109 yeast strain and...
<p>The coding sequence for full length wild type SIRT6 and the H133W mutant was PCR-amplified and cl...
<p>(A) A yeast two-hybrid assay showing interaction between Gm08DWD and GmMYB176 Yeast cells were co...
<p>The yeast strains AH109 were co-transformed with various BD and AD fusion constructs as indicated...
<p>Proteins of interest were fused with Gal4 DNA binding domain (BD, left) and with Gal4 activation ...
<p>OsMKK3, OsMKK4, OsMKK6 and OsMKK10-2 were fused with GAL4 DNA-binding domain and were used as a b...
<p>(A) Schematic diagram of mutant and wild type of HbFd1. HbFd1△C1 represents deletion of 10 amino ...
<p>(A) Schematic representation of the different Gal4-DBD-PR-Set7 bait proteins used for the yeast t...
<p>Shown are serial dilutions of diploid yeast strains obtained after mating and spread on SD medium...
<p>(A) Yeast two-hybrid assay. The combinations of the plasmids used for transformation of the yeast...
<p>The yeast two-hybrid interaction between <i>Pm</i>Fortilin fragments and FBP1 in <i>S.cerevisiae<...
<p>(A) A Y2H assay was used to detect the interactions of MED8 with the MYC2 protein. Yeast cells co...
<p>Yeast cells containing the indicated plasmids were serially diluted, and 5 µl of diluted cells wa...
<p>The yeast strain YH2Gold containing the indicated plasmid combinations was grown on nutritional s...
<p>Transformants containing the bait and prey plasmids were visualized by their growth on double dro...
<p>The pGBKT7-TaCBLs and pGADT7-TaCIPK2 plasmids were co-transformed into the AH109 yeast strain and...
<p>The coding sequence for full length wild type SIRT6 and the H133W mutant was PCR-amplified and cl...
<p>(A) A yeast two-hybrid assay showing interaction between Gm08DWD and GmMYB176 Yeast cells were co...
<p>The yeast strains AH109 were co-transformed with various BD and AD fusion constructs as indicated...
<p>Proteins of interest were fused with Gal4 DNA binding domain (BD, left) and with Gal4 activation ...
<p>OsMKK3, OsMKK4, OsMKK6 and OsMKK10-2 were fused with GAL4 DNA-binding domain and were used as a b...
<p>(A) Schematic diagram of mutant and wild type of HbFd1. HbFd1△C1 represents deletion of 10 amino ...
<p>(A) Schematic representation of the different Gal4-DBD-PR-Set7 bait proteins used for the yeast t...
<p>Shown are serial dilutions of diploid yeast strains obtained after mating and spread on SD medium...
<p>(A) Yeast two-hybrid assay. The combinations of the plasmids used for transformation of the yeast...
<p>The yeast two-hybrid interaction between <i>Pm</i>Fortilin fragments and FBP1 in <i>S.cerevisiae<...
<p>(A) A Y2H assay was used to detect the interactions of MED8 with the MYC2 protein. Yeast cells co...
<p>Yeast cells containing the indicated plasmids were serially diluted, and 5 µl of diluted cells wa...
<p>The yeast strain YH2Gold containing the indicated plasmid combinations was grown on nutritional s...
<p>Transformants containing the bait and prey plasmids were visualized by their growth on double dro...
<p>The pGBKT7-TaCBLs and pGADT7-TaCIPK2 plasmids were co-transformed into the AH109 yeast strain and...