Limit-of-detection assay.

<p>Two samples with known mutations were mixed at different percentages, captured and sequenced. VAFs of each mutation were plotted against the percentage in the mix. (A) Mutations KRAS G13D (58%), FBXW7 R505S 48%), APC K1462fs (52%), NRAS Q61R (35%) with high VAFs in the original sample. (B) Mutations NRAS Q61L (9%), ERBB2 V842I (8%), TP53 Y234* (9%), KRAS D12D (24%) with low VAFs in the original sample.