Maternal-of-origin-dependent imprinted methylation marks at the <i>WRB</i> CGI-2 DMR.

<p>5^mCpG-sensitive restriction endonuclease sites at the <i>WRB</i> CGI-2 DMR are differentially methylated on the maternal allele <i>versus</i> the paternal allele in a manner consistent with imprinting. Electropherograms of the genotype profiles in a control nuclear family, informative for the rs2244352 (C>A) SNP neighboring the <i>WRB</i> CGI-2 DMR. In the child, the maternal-derived C allele is 100% resistant to <i>Hha</i>I digestion (fully methylated), whereas the paternal-derived A allele is 100% susceptible to <i>Hha</i>I digestion (i.e., unmethylated). The parental allele-specific methylation statuses were validated using the <i>McrBC</i> restriction endonuclease that cleaves methylated DNA and, therefore, the unmethylated paternal allele, but not the maternally methylated allele, remains undigested.