Fluorescence emission spectra of ThT (20 μM; curve 1) and ThT solution incubated with heated BSA (0.4 mg/ml) for 30 min at 25°C.

<p>BSA (1 mg/ml) was preincubated for 12 h at 60°C, 65°C, 70°C and 80°C (curves 2–5, respectively). Excitation wavelength was 450 nm.