Characterization of activated human T cell-released exosomes.

<p>(A) Study design for the preparation of activated human T cell-released exosomes. Day 14 culture supernatant of OKT3-, RetroNectin- and rIL-2-activated human PBMCs were collected and subjected to ultracentrifugation after removing cell debris and protein aggregations. (B) Day 14 OKT3-, RetroNectin- and rIL-2-activated human PBMCs were examined for CD4 and CD8 expression using flow cytometry. (C) Mean diameter of the activated human T cell-derived exosomes, measured by NanoSight LM10. (D) Expression of tetraspanins (CD9, CD63, and CD81), T cell markers (CD3, CD4, CD8, CD28, CD45, and pan-TCRαβ) and HLAs (pan-HLA-I and pan-HLA-II) of the day-14 activated human T cells (Donor T cell) or their released exosome bound with latex beads (Exo) were determined by flow cytometry after staining with molecule-specific monoclonal antibodies or isotype controls.