The global motions and exchange kinetics of a model protein, ubiquitin, bound to the surface of negatively charged lipid-based nanoparticles (liposomes) are derived from combined analysis of exchange lifetime broadening arising from binding to nanoparticles of differing size. The relative contributions of residence time and rotational tumbling to the total effective correlation time of the bound protein are modulated by nanoparticle size, thereby permitting the various motional and exchange parameters to be determined. The residence time of ubiquitin bound to the surface of both large and small unilamellar liposomes is ∼20 μs. Bound ubiquitin undergoes internal rotation about an axis approximately perpendicular to the lipid surface on a low...
Understanding how proteins function at the atomic level relies in part on a detailed characterizatio...
Understanding the binding of nanoparticles to receptors on biomembranes is critical to the developme...
Lipids and proteins, as essential components of biological cell membranes, exhibit a significant deg...
A solution-state NMR method is proposed to investigate the dynamics of proteins that undergo reversi...
A solution-state NMR method is proposed to investigate the dynamics of proteins that undergo reversi...
The dynamics of molecules in solution is usually quantified by the determination of timescale-specif...
Understanding how proteins function at the atomic level relies in part on a detailed characterizatio...
To probe internal motions in proteins on the 10−8−10−5 s time scale by NMR relaxation, it is necessa...
International audience ; Understanding how proteins function at the atomic level relies in part on a...
A 0.2 mu s molecular dynamics simulation of ubiquitin in water is presented, which allows us to asse...
International audienceProteins perform their functions in solution but their structures are most fre...
The internal motions of biomolecules were examined by analyzing NMR relaxation in terms of the model...
International audience: Understanding the molecular determinants underlying protein function require...
Since many biological processes occur on the μs to ms time scale, internal dynamics on that time sca...
We demonstrate that conformational exchange processes in proteins on microsecond-to-millisecond time...
Understanding how proteins function at the atomic level relies in part on a detailed characterizatio...
Understanding the binding of nanoparticles to receptors on biomembranes is critical to the developme...
Lipids and proteins, as essential components of biological cell membranes, exhibit a significant deg...
A solution-state NMR method is proposed to investigate the dynamics of proteins that undergo reversi...
A solution-state NMR method is proposed to investigate the dynamics of proteins that undergo reversi...
The dynamics of molecules in solution is usually quantified by the determination of timescale-specif...
Understanding how proteins function at the atomic level relies in part on a detailed characterizatio...
To probe internal motions in proteins on the 10−8−10−5 s time scale by NMR relaxation, it is necessa...
International audience ; Understanding how proteins function at the atomic level relies in part on a...
A 0.2 mu s molecular dynamics simulation of ubiquitin in water is presented, which allows us to asse...
International audienceProteins perform their functions in solution but their structures are most fre...
The internal motions of biomolecules were examined by analyzing NMR relaxation in terms of the model...
International audience: Understanding the molecular determinants underlying protein function require...
Since many biological processes occur on the μs to ms time scale, internal dynamics on that time sca...
We demonstrate that conformational exchange processes in proteins on microsecond-to-millisecond time...
Understanding how proteins function at the atomic level relies in part on a detailed characterizatio...
Understanding the binding of nanoparticles to receptors on biomembranes is critical to the developme...
Lipids and proteins, as essential components of biological cell membranes, exhibit a significant deg...