<p>ELISA signal intensity following incubation of bacterial lysates obtained from <i>E</i>. <i>coli</i> cells overexpressing WT or D355N mutant 3XMBT with RelA methylated (black) and un-methylated (grey) peptides. Data are representative of three independent experiments (error bars, S.D.).</p
<div><p>(<b>A</b>) Amino acid sequence alignment between segments of M.HpaII and M.SssI. Conserved r...
<p>A. Nucleotide sequences and the deduced amino acid sequences of the encoded peptide fusions to C-...
<p>As a negative control <i>E. coli dsbA</i>::<i>aph</i> was transformed with an empty pHEL2 vector....
<div><p>The ε-amino group of lysine residues may be mono-, di- or tri-methylated by protein lysine m...
<p><b>Structural basis for the high specificity of T411L 3XMBT mutant toward di-methyllysine (A)</b>...
<p>On the X-axis, a dilution factor of 0 is the highest concentration of enzyme and corresponds to t...
<p>*Methylated residue indicated in bold.</p><p>**Exclusively detected in wild type cells.</p><p>Lys...
<p>The dilution series and presentation of data are the same as in <a href="http://www.plosone.org/a...
<p>A. The plasmids pWHU668-pWHU674 possessing mutated lysine residues were expressed in <i>E. coli</...
<p><i>Lane M</i>, molecular weight marker; <i>lane 1</i>, unmethylated plasmid pHCMC04; <i>lane 2</i...
<p>(A) Relative luminescence intensity of WT, <i>rpt2a-2</i>, <i>met1-1</i> and <i>rpt2a-2met1-1</i>...
<p>(A) <i>in vitro</i> methylation assay. Bacterial expressed GST-FUS or GST alone (control) was add...
An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employ...
<p>(<b>A</b>) The clones with the highest signal on immobilized lysozyme are shown in the figure. Th...
While a number of post-translational modifications (PTM), such as phosphorylation and ubiquitination...
<div><p>(<b>A</b>) Amino acid sequence alignment between segments of M.HpaII and M.SssI. Conserved r...
<p>A. Nucleotide sequences and the deduced amino acid sequences of the encoded peptide fusions to C-...
<p>As a negative control <i>E. coli dsbA</i>::<i>aph</i> was transformed with an empty pHEL2 vector....
<div><p>The ε-amino group of lysine residues may be mono-, di- or tri-methylated by protein lysine m...
<p><b>Structural basis for the high specificity of T411L 3XMBT mutant toward di-methyllysine (A)</b>...
<p>On the X-axis, a dilution factor of 0 is the highest concentration of enzyme and corresponds to t...
<p>*Methylated residue indicated in bold.</p><p>**Exclusively detected in wild type cells.</p><p>Lys...
<p>The dilution series and presentation of data are the same as in <a href="http://www.plosone.org/a...
<p>A. The plasmids pWHU668-pWHU674 possessing mutated lysine residues were expressed in <i>E. coli</...
<p><i>Lane M</i>, molecular weight marker; <i>lane 1</i>, unmethylated plasmid pHCMC04; <i>lane 2</i...
<p>(A) Relative luminescence intensity of WT, <i>rpt2a-2</i>, <i>met1-1</i> and <i>rpt2a-2met1-1</i>...
<p>(A) <i>in vitro</i> methylation assay. Bacterial expressed GST-FUS or GST alone (control) was add...
An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employ...
<p>(<b>A</b>) The clones with the highest signal on immobilized lysozyme are shown in the figure. Th...
While a number of post-translational modifications (PTM), such as phosphorylation and ubiquitination...
<div><p>(<b>A</b>) Amino acid sequence alignment between segments of M.HpaII and M.SssI. Conserved r...
<p>A. Nucleotide sequences and the deduced amino acid sequences of the encoded peptide fusions to C-...
<p>As a negative control <i>E. coli dsbA</i>::<i>aph</i> was transformed with an empty pHEL2 vector....