<p>Expression of IFITM proteins was induced in Vero and 293T cells after treatment with Universal type-I IFN at both 1,000 or 10,000 U/ml concentrations for 24 h and compared with untreated cells. Total cell extracts were incubated with antibodies against IFITM1 <b>(</b>A), IFITM2 (B) and IFITM3 (C). Right side panels represent WB quantification by densitometry for each IFITM and are expressed as the mean±SD from three independent experiments. Statistical significance was evaluated by a one-way ANOVA followed by Bonferroni’s multiple comparison test. Differences are marked with asterisks as indicated (**<i>p</i><0.01; ***<i>p</i><0.001).</p
<p>(<b>A</b>) KT1, K562, KCL22 and BV173 cells were treated with or without 1000 IU/ml IFNα2a for 2 ...
<p>Serum was collected from wild-type (<b>A-F</b>), <i>Ifnar1</i><sup>-/-</sup> (<b>G-L</b>), <i>Ifn...
<p>Single cell suspensions of splenocytes or lung mononuclear cells were prepared at day 14 post-inf...
<p>(A) K562 cells were treated with the indicated concentrations of human interferon-α or mock treat...
(A) Western blot analysis of IFITM3 expression by CD8+ T cells before (0) and after in vitro activat...
Using a specific antibody, we found that expression of the viral restriction factor IFITM3 differs a...
<p>Results shown are of fold increase in IFN-α and IFN-β transcript levels (measured by real-time PC...
<p>Log ratio: Different in expression level between acute and convalescent samples, a value of 0.3 i...
<p>WT, <i>Irf3<sup>−/−</sup>×Irf7<sup>−/−</sup></i> DKO, <i>Irf3<sup>−/−</sup>×Irf5<sup>−/−</sup>×Ir...
LAB genomes were introduced into HT-29 cells using lipofection. After 24 h, the medium was collected...
<p>Immunofluorescence of A549 cells expressing HA-tagged IFITM1, IFITM2 and IFITM3 (or control A549 ...
<p>Ifitm3 protein expression can also be induced by gp130-family cytokines in cells expressing appro...
<p>(A) A549 cells transduced with the vector alone or with tet-inducible native FL IFITM3 or Δ21 IFI...
<p>Mice were inoculated with 10<sup>2</sup> PFU of WNV by footpad injection and sacrificed at the in...
<p>(A). Western blot of Vero cell lines expressing IFITM1, 2 and 3. (B). Graphics depict mean±SD of ...
<p>(<b>A</b>) KT1, K562, KCL22 and BV173 cells were treated with or without 1000 IU/ml IFNα2a for 2 ...
<p>Serum was collected from wild-type (<b>A-F</b>), <i>Ifnar1</i><sup>-/-</sup> (<b>G-L</b>), <i>Ifn...
<p>Single cell suspensions of splenocytes or lung mononuclear cells were prepared at day 14 post-inf...
<p>(A) K562 cells were treated with the indicated concentrations of human interferon-α or mock treat...
(A) Western blot analysis of IFITM3 expression by CD8+ T cells before (0) and after in vitro activat...
Using a specific antibody, we found that expression of the viral restriction factor IFITM3 differs a...
<p>Results shown are of fold increase in IFN-α and IFN-β transcript levels (measured by real-time PC...
<p>Log ratio: Different in expression level between acute and convalescent samples, a value of 0.3 i...
<p>WT, <i>Irf3<sup>−/−</sup>×Irf7<sup>−/−</sup></i> DKO, <i>Irf3<sup>−/−</sup>×Irf5<sup>−/−</sup>×Ir...
LAB genomes were introduced into HT-29 cells using lipofection. After 24 h, the medium was collected...
<p>Immunofluorescence of A549 cells expressing HA-tagged IFITM1, IFITM2 and IFITM3 (or control A549 ...
<p>Ifitm3 protein expression can also be induced by gp130-family cytokines in cells expressing appro...
<p>(A) A549 cells transduced with the vector alone or with tet-inducible native FL IFITM3 or Δ21 IFI...
<p>Mice were inoculated with 10<sup>2</sup> PFU of WNV by footpad injection and sacrificed at the in...
<p>(A). Western blot of Vero cell lines expressing IFITM1, 2 and 3. (B). Graphics depict mean±SD of ...
<p>(<b>A</b>) KT1, K562, KCL22 and BV173 cells were treated with or without 1000 IU/ml IFNα2a for 2 ...
<p>Serum was collected from wild-type (<b>A-F</b>), <i>Ifnar1</i><sup>-/-</sup> (<b>G-L</b>), <i>Ifn...
<p>Single cell suspensions of splenocytes or lung mononuclear cells were prepared at day 14 post-inf...