Analysis of apoptosis by Cleaved Caspase III (CC3) staining.

<p>Apoptotic levels were determined by cleaved caspase III staining. A) Representative images of TEBs stained for CC3 and EdU incorporation. B) Quantification of CC3 positive cells indicates the majority of apoptosis occurs in Regions 5, 6, and 7 (n = 50 TEBs). See also <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1004839#pcbi.1004839.s004" target="_blank">S4 Fig</a>.