Effects of PGE₂ on beige/brite differentiation.

<p><b>(A)</b> Adipose tissue expression of UCP1 in adipose tissue from non-obese control individuals (n = 10) and obese patients (n = 11). <b>(B)</b> Expression of UCP1 and PRDM16 in obese fat pads incubated with vehicle (Veh, 0.19% DMSO) or PGE₂ (1 μM) for 24 hours. <b>(C)</b> Representative photomicrographs of stromal vascular cell (SVC) and adipocyte cultures isolated from adipose tissue of obese patients (x200 magnification; scale bars; 200 μm). <b><i>Right panel</i></b>: Expression of COX-2 and mPGES-1 in SVC and adipocytes from obese adipose tissue. <b>D)</b> Expression of UCP1 and PRDM16 in differentiated pre-adipocytes isolated from obese patients growing in the presence of Veh (0.19% DMSO) or PGE₂ (1 μM). <b>(E)</b> Expression of UCP1 and PRDM16 in adipocytes isolated from obese adipose tissue and incubated with Veh (0.19% DMSO) or PGE₂ (1 μM) for 3 h. <b>(F)</b> Representative western blot of phosphorylated HSL (pHSL) protein expression in adipose tissue from non-obese control individuals (CT) (n = 7) and obese patients (Ob) (n = 8). <b>(G)</b> Glycerol levels released by adipocytes incubated with vehicle (V) (0.19% DMSO) or isoproterenol (1 μM) in the presence of V or PGE₂ (1 μM) for 1.5 h. The results are expressed as mean ± SEM. *, P<0.05 versus control or Veh; a, P<0.05 and b P<0.01 versus SVC. #, P<0.05 versus isoproterenol-treated adipocytes.