Expression of TGFβ1 and RASAL1 by qPCR and Western blotting in GTM cells compared to NTM cells.

<p><b>A.</b> mRNA expression of TGFβ1 and RASAL1 by qPCR. There was no change in mRNA expression of TGFβ1 in GTM cells; 0.98+/-0.09 fold change. RASAL1 showed decreased mRNA expression in GTM cells; 0.53+/-0.06 (P<0.01). <b>B.</b> We examined the protein expression of TGFβ1 and RASAL1 by Western blotting and conducted densitometry on the blots. TGFβ1 protein expression was increased in GTM cells compared to NTM cells. RASAL1 showed decreased protein expression in GTM cells compared to NTM cells. n = 3 **P<0.01. Results shown are from 3 normal and 3 glaucoma donors with each performed in triplicate. (donor cell strain shown).